Sentence examples for individuals was digested from inspiring English sources
Genomic DNA from each of the 24 library individuals was digested separately for 30 minutes at 37°C, in 50 µL reactions with 15 units of SbfI (NEB).
RAD-seq libraries were constructed using a protocol adapted from Baird et al. Briefly, 250 ng of genomic DNA from each of 218 library individuals was digested separately with 20 units of PstI and then heat inactivated at 65°C.
DNA from these individuals was digested with the restriction enzyme AfaI (Invitrogen, Carlsbad, California, USA), and the fragments were linked to Afa21 (5′-CTCTTGCTTACGCGTGGACTA-3′) Afa25fa25′-CTCTTGCTTACGCGTGGACTA-3′-3′) adapters.
To investigate copy number variation of monomers within the SST1 macrosatellite arrays, agarose embedded DNA from 22 unrelated individuals was digested with XbaI that does not cut within the array and separated by PFGE before Southern blotting and hybridization with a probe specific to the SST1 arrays.
A total of 92 individuals were digested with the restriction enzyme pair PstI MluI, and 182 individuals were used to prepare the PstI-MseI complexity reduced library.
Genomic DNAs from the PER-453 cell line and PBLs from two normal individuals were digested with EcoRI and probed with a FOXO1A cDNA probe.
Genomic DNA of each B- and R- individual was digested with EcoRI.
DNA from one individual was digested separately with RsaI (New England Biolabs, Ipswich, Massachusetts, USA) and AluI (New England Biolabs).
To summarize, DNA from each individual was digested with SbfI and then barcoded with a unique 6-base sequence 3′ to the SbfI cut site.
In short, genomic DNA for each individual was digested with restriction enzyme EcoRI, ligated to a modified Illumina P1 adapter containing individual-specific barcodes and sheared to an average size of 500 bp.
100 ng of genomic DNA from each individual was digested in a 50 μL reaction with 100 units each of NlaIII and MluCI restriction enzymes (New England Biolabs, Beverly MA, USA), NEB Buffer 4, BSA and water for 3 hrs at 37°C, without a heat kill step.
