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Here, we present a method where PCR products are used to capture targets for sequencing from pooled sequencing libraries of multiple individuals, using standard laboratory equipment.
BMI and blood pressure were measured by trained individuals using standard operating procedures.
It was used to amplify products from genomic DNA of homozygous cases, carriers and unaffected unrelated individuals using standard procedures.
The cytokinesis-block micronucleus assay was performed on whole blood cultures from 100 individuals using standard procedures [ 50].
The antihypertensive effects of aerobic exercise have been well established in both normotensive and hypertensive individuals using standard brachial sphygomanometry [ 29].
These coalescent simulations generated topologies and coalescent times for 5000 individuals using standard approaches (Hudson 1990); however, inversion and nucleotide mutations were generated on the branches of the tree rather than just nucleotide changes.
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Note that since there is no replication per se, each individual is unique and each brain region is unique per individual, using standard t-tests or one-way ANOVAs for comparison of gene expression is not applicable to this data set.
All tests were administered by the same individual using standard techniques and under the same environmental conditions.
Table 3 contains an example of the mapping of an assay ratio of 0.675 onto a grid representing the penetrance function for an individual, using standard deviations for a CV of 3%.
Genomic DNA was extracted from blood (53 individuals) or brain [fresh and/or formalin-fixed, paraffin-embedded (six individuals)] using standard protocols (Murrell et al., 1991).
Participants either conducted the individual scenarios using standard maritime protocol (i.e. all comms carried out over UHF radio), or used the prototype PPU device to facilitate an understanding of relative position between tugs and vessel.
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