The individual retention times of bilobalide and ginkgolides were compared with that of standards.
Furthermore, considering the structural differences between charge variants of a monoclonal antibody, it was possible to predict their individual retention times.
Chromatographic conditions were optimised and the best separation, with individual retention times for most of the analytes, was obtained on a Zorbax SB-CN column within a 9-min gradient run.
The specific magnitudes and significance of the individual retention processes will depend upon the properties and conditions of the specific system of interest (e.g., PFAS constituent and concentration, porous medium, aqueous chemistry, fluid saturations, co-contaminants).
A chromatographic response function (CRF) which included the number of peaks, the resolution between adjacent peaks, a specified analysis time, and the individual retention times relative to a minimum retention time, was calculated to evaluate the quality of the individual chromatograms.
Each TIC mass spectrum for a ferulic acid ((M-H −, 193.2), b vanillin ((M-H −, 193.2), c vanillic acid ((M-H)−, 167.1), d vanillin glucoside ((M-H)−, 313.3) and individual retention times were identical to those of the authentic compounds Fig. 3 The mass spectrometry of vanillin glucoside produced during the phenolic biotransformation of Aspergillus luchuensis.
Pigments (n = 15, listed in the legend of Figure 1) were identified by comparing their individual retention times to those of commercially available pigment standards; Chlorophyll a and Chlorophyll b standards, Sigma Chemical Co. and a Pigmix standard, containing 20 pigments, Water Quality Institute (VKI), Hørsholm, Denmark.
Clicking on aggregated retention factors themselves retrieves a summary of the individual retention factors that have been averaged, along with corresponding details and the original reference.
Where aggregated retention factors are displayed, the value itself can be clicked to retrieve details of each individual retention factor used in the aggregation.
The wide range of compounds included in this study with individual retention times demonstrates the potential of this semi-preparative method.
