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Females were pooled into two samples, each with 30 individuals (one sample per ecotype).
Liver and spleen were collected from both individuals (one sample from each) while one sample from one individual was collected from kidney, head-kidney, heart, brain, gills, white muscle and intestine.
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For some traits, particularly ones that rely upon qualitative diagnosis, care is taken to ensure that the same diagnostic criteria are used [ 5], but there is always the possibility that different individuals will interpret the criteria in their own way, leading to heterogeneity in classifying individuals from one sample to the next.
An increasingly popular approach to characterizing the genetic variation in a population involves pooling DNA from a large number of individuals into one sample from which a single DNA library is extracted.
Of 9,739 individuals screened, one sample was confirmed as a double recombinant across the XR inversion, with the D. pseudoobscura allele at the center of the inversion and the D. persimilis allele just inside both breakpoints.
The discrimination group was presented with 2 different samples from one individual and one sample from a different individual.
Briefly, an equimolar amount of DNA was taken from every individual from one sample set and put into a single tube (one pool).
All individuals pooled into one sample were from the same colony.
45, was a deletion present in only 2/351 individuals and only one sample was available for validation.
To demonstrate this, we analyzed an in-house RNA-seq dataset (unpublished) from human postmortem brain tissue obtained from two individuals with schizophrenia: one sample has an acceptable RIN (=7.8) and the other sample has a very low RIN (=3).
An identical mitochondrial DNA sequence was obtained from the six golden strain individuals, one of the samples we used for RAD tag sequencing (a female), 20 of the samples we used for PCR screens (9 females and 11 males), and one individual sampled from Nigeria.
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