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Notably, certain high-risk individuals (described previously) would benefit from a physician visit and diagnosis rather than self management of influenza-like symptoms.
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Genomic DNA was extracted from EDTA-preserved peripheral venous blood from all individuals, as described previously [ 25].
Primary human mammary cells have been isolated from cryopreserved biopsies of two individuals as described previously [ 21].
Genomic DNA was extracted from frozen EDTA preserved peripheral venous blood from all individuals, as described previously [ 55].
Total keratinocytes were obtained from normal human epidermis derived from young or adult individuals as described previously (Marconi et al, 2004).
These adjustment factors were determined by simulation on the basis of the known relatedness of the individuals as described previously (32).
Non-transformed CD4+ve T cell lines were generated by phytohaemagglutinin (PHA) stimulation of CD4+ve T cells isolated by negative selection from the peripheral blood of normal individuals as described previously [ 3].
The PPA and FFA were defined by a separately conducted localizer scan performed for each individual as described previously.
Slides were examined in a blind fashion by an experienced individual as described previously (13).
The breeding scheme for producing BC2F1 individuals has been described previously [ 51].
A significant decrease in macrophage precursors and macrophages in the bone marrow of elderly individuals has been described previously [ 10].
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