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Blots were individually immunoblotted.
Immunoblots were transferred to PVDF membranes and were individually immunoblotted with anti-Ub antibody (Life Sensors).
Blots were individually immunoblotted with anti-Flag monoclonal antibodies (Sigma Aldrich).
Blots were individually immunoblotted with anti-HA antibody (Santa Cruz Biotechnology).
Gels were transferred and were individually immunoblotted with anti-Myc monoclonal antibodies (Abcam).
Gels were transferred and individually immunoblotted with anti-Myc antibody (Abcam) or anti-Flag antibody (Sigma).
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We next conducted IP's for both LEF-1 and TCF-4 proteins individually and immunoblotted for the presence of β-CATENIN protein levels.
The respiratory complexes and supercomplex bands separated by 2D BN/SDS/PAGE were immunoblotted individually with antisera to components of the respiratory complexes.
To test this hypothesis, we independently co-transfected each single Cdk site mono-phosphorylated Rb-HA construct and control un-phosphorylatable ΔCdk Rb-HA into cells co-expressing the E1a oncoprotein or E2F-1, E2F-3, E2F-3, E2F-4 transcription factors (Myc tagged), then individually immunoprecipitated E1a and each E2F, and immunoblotted for the associated Rb mono-phosphorylated isoforms.
Total Rip2 was immunoblotted as a control.
Each fractionation was then immunoblotted.
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