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A multiplex-nested PCR system was optimized by varying the following individually: concentrations of Mg2+ and dNTPs, ratio of concentrations of primers and numbers of cycles.
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In addition, the following basic goodness-of-fit plots were used for diagnostic purposes (i) observed vs. individually predicted concentrations, (ii) observed vs. population-predicted concentrations, (iii) conditional weighted residuals vs. time, and (iv) conditional weighted residuals vs. population-predicted concentrations.
Alpha-pinene, β-pinene, and limonene (Sigma) were tested individually at concentrations ranging from 0.05% to 1.0% v/v with 2 5 replicates.
In all cases, when each compound was applied individually, higher concentrations (viz., higher MICs) were required for the complete inhibition of fungal growth compared to the combination of all compounds in formulations.
Each PCR reaction component, including magnesium, biotin and primer pool concentration, was tested individually at various concentrations.
First, chemical compounds (glycerol, uracil, thymine, uridine, thymidine; solubilized in tap water filtered through Whatman No. 1 cellulose filter paper (Millipore, Saint-Quentin-en-Yvelines, France)) were tested individually at different concentrations to determine the attractive concentration.
Laboratories were able to refine the dosing and individually determine suitable concentrations of test substances, according to their experimental set up.
We weigh the available human evidence focusing on EDCs and endometriosis, restricting to research that has individually quantified chemical concentrations for women, included a comparison group of unaffected women, and used multivariable analytic techniques.
Moreover, chemical analysis allowed us to identify a certain number of commonly found, highly water-soluble, low-molecular-weight compounds, several of which (glycerol, uracil, thymine, uridine, thymidine) were able to induce attraction when tested individually but at concentrations substantially higher than those found in nature.
The experiments were conducted by adding each of the substrates individually, at various concentrations to study substrate inhibition.
For subsequent combination studies, a concentration of 400 μM TMZ plus 75 μM Qct was used as those concentrations individually resulted in a distinguishable degree of apoptotic cell death.
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