Twenty crossed (Bos taurus×Bos indicus) steers (270±30 kg BW) were used in a grazing trial (80 days), feeding individually concentrate (1 kg/d, 14% CP) and urea treated sugar cane (USC), in a completely randomized design according to the following treatments: (1) Grazing control (GC); (2) USC-0; (3) USC+15 g d−1 Fibrozyme (USC-15); (4) USC+30 g d−1 Fibrozyme (USC-30).
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To identify differences between these two types of EV71 viral particles, fractions 10 and 16 were individually diafiltrated with PBS and concentrated to 5 mL as virus stocks.
During the experiment (d 0 203), 40 multiparous, nonpregnant, lactating 3/4 Holstein × 1/4 Gir cows (initial days in milk = 81±± 2; mean ± SE) were offered corn silage for ad libitum consumption, and individually received concentrate formulated to allow diets to provide 160% of their daily net energy for lactation requirements.
Simmental × Angus: 1,445 Simmental-sired steers originating from 6 ranches were individually fed concentrate rations at the University of Illinois at Urbana-Champaign using a GrowSafe system in 2005 (N =231), 2006 (N =320), 2007 (N =322), 2008 (N =347) and 2009 (N =225).
The total estimated dry matter intake (EDMI, kg/day) was calculated by adding the individually recorded concentrate dry matter intake (DMI) (kg/day) to the corrected TMR dry matter intake (kg/day) using equation (4) according to Kristensen and Ingvartsen (2003).
Each well was extracted individually and, following isolation, two wells were pooled and concentrated to half the original volume using a vacuum concentrator (Vacufuge Concentrator 5301; Eppendorf).
Following the labeling reaction, the individually labeled test and reference samples were concentrated using Microcon YM-30 filters (Millipore, Billerica, MA) and then combined.
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