To address the question, these amino acid residues in human ODC were individually changed to the residues present in human AZI.
Using site-directed mutagenesis, His, Gly, Gly, Ser and Pro were individually changed to alanine or cysteine.
Each of the seven cysteines (the four conserved residues, Cys32, Cys61, Cys156, and Cys206, and nonconserved, Cys72, Cys85, and Cys250) was individually changed to serine.
The individually changed proteins at a specific time point refer to the proteins that showed significant alterations in their abundances at this single time point, while the cumulatively changed proteins at the same time point refer to a collection of individually changed proteins from day 26 up to this time point.
To illustrate this potential, we measured receptor activation on a series of SST-14 mutant peptides, in which amino acids at positions 6 10 were individually changed to alanine.
True confounders were identified by sequentially including each confounder in the regression model, and including in the multiple variable regression those confounders that individually changed the estimation after adjustment by more than 10%.
For the final multivariable model, we included variables that individually changed the effect estimate by at least 3%% (n = 9) and added additional variables that changed the estimate by 1%% by applying a forward selection procedure.
A final model was then developed by including all additional confounders that individually changed the effect of interest on the attained BMI level at age 10 (level) or the rate of change in BMI levels (slope) by at least 10%.
