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Finnish (n = 101) and Swedish (n = 134) patients positive for Ro/SSA-autoantibodies were first analysed individually and further in combination (n = 235) as a pooled dataset in order to study the association between ITGAM and immunological factors [12], [26].
ASMS markers (Table 1) were amplified individually and further pooled for separation and detection on an ABI 3130xl genetic analyzer (Applied Biosystems), while ASMS2012 markers were multiplexed for amplification in two triplex reactions.
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The outliers were then individually selected and further studied to define the relationship.
Hygromycin-resistant colonies for piggyBac and Tol2 were individually cloned and further expanded.
Clones were individually picked and further characterized for the expression of the Fucci reporter proteins.
Our concept allows direct mapping of the dietary effects on molecular mechanisms in the three tissues individually, and by further linking concerted dietary effects in all three tissues combined, gaining insight into how nutrition influences whole body metabolism.
As shown in Figure 7, compared with the control, the cell numbers of the 3 human cancer cell lines decreased remarkably after treatment with either TC extract or 5-FU individually and even further after treatment with the combination.
The selected phage clones (10 pfu/well) and the negative control, M13KE phage (New England Biolabs) were added individually onto the cells and further incubated at 37 °C for 1 h.
All coated wells were washed with PBST; 200 μL of a blocking solution (1% BSA in PBS, pH 7.4) was added individually and the plate was further incubated at 37°C for 1 hr.
Identified proteins were sent to the Proteinscape™ database (version 1.3, Protagen, Dortmund, Germany) and checked individually for further consideration.
If we account for both linear and non-linear connections individually, a further two connections are not discovered (linear to and non-linear to ).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com