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The individualized cells were placed in MethoCult (Stem Cell Technologies) media for quantifying colony-forming units per the manufacturer's instructions.
We evaluated the self renewal properties of H1650-ER1 cells by the ability of individualized cells to form spheroids when seeded in agarose and cultured in serum free medium supplemented with EGF and bFGF.
Phospho-Erk1/2 proteins in this cell line were restricted to the cell membrane in areas of cell-to-cell contact at the periphery of large multicellular clusters (see Additional File 4, Figure S2), and were not seen in individualized cells or in cells forming smaller clusters, suggesting activation was probably mediated by intercellular signaling.
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But he said that in practice, therapeutic cloning would "never, ever fly" because it is individualized cell therapy, not mass production, that is needed for cell therapies to succeed commercially.
Although cellular immunotherapy based on autolgous dendritic cells (DCs) targeting antigens expressed by metastatic cancer has demonstrated clinical efficacy, the logistical challenges in generating an individualized cell product create an imperative to develop alternatives to DC-based cancer vaccines.
However that may be, conditionally immortalized CD40-stimulated B cells present new opportunities to study B cell function and differentiation, and they offer themselves as tools for individualized cell or gene banking and for use in cellular immunotherapy.
Individualized cell based subtelomeric profile will lead to design a reliable signature of subtelomeric p/q ratio for SCN and SI in BUC and ALN cells.
Jenq, R.R. & van den Brink, M.R. Allogeneic haematopoietic stem cell transplantation: individualized stem cell and immune therapy of cancer.
In Drosophila, at the end of spermatogenesis, a process known as sperm individualization generates 64 mature and individualized sperm cells within a cyst.
The extensive exon-based sequencing of tumors to identify mutations has opened up the possibility of individualized T cell receptor (TCR) T cell therapy targeting neo-antigens [ 150].
Distinctively more antigens were detected in the autologous combinations of proteomes and sera than in the heterogeneous suggesting an individualized tumor cell antigenicity.
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