Exact(6)
Individualized alignment also improves read alignment accuracy and abundance estimates in the DO sample.
Individualized alignment improves the mapping resolution of local eQTL.
Moreover, when applied to expression QTL mapping we find that our individualized alignment strategy corrects false-positive linkage signals and unmasks hidden associations.
Individualized alignment of liver RNA-seq data from CAST and DO samples yields alignment statistics that are similar to our simulation results; this suggests that improvements in mapping accuracy and gene abundance estimates are also similar to the simulation results.
Protein-coding genes are the predominant gene biotype in the simulated data set and as such account for most of the eQTL call improvements observed from the individualized alignment strategy (Table S7).
In the comparison of gene abundance estimates, we find that a total of 714 expressed genes differ by >10% from alignment strategy alone, and most of these (432 of 714 genes) show more accurate estimates from individualized alignment in the simulation study (green circles in Figure 3C).
Similar(53)
Among the 6097 local eQTL that are identified by both alignment strategies, most increase in significance from individualized alignments.
These genes appear to be highly sensitive to alignment strategy; the median difference in abundance estimates between NCBIM37 and individualized alignments was 16% in the real data and 14% in the simulated data (green bars in Figure 3D).
The dynamic PET images (2 × 2 × 2 mm) were initially motion corrected by rigidly registering each frame to a reference frame (13 15 min p.i). using mutual information and subsequently rigidly transformed into alignment with the MRI and individualized atlas.
It is individualized.
"Education should be individualized.
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