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Genomic DNA samples from each individual were quantified using spectrophotometry (Nanodrop) and checked for genomic integrity by agarose gel electrophoresis.
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The behaviour of each individual was quantified using a probabilistic stimulus/response function.
SNP frequencies and overlap among individuals were quantified using custom Perl scripts.
For each transmission pair, selection within individuals was quantified using the ratio of nonsynonymous to synonymous substitutions (ω = dN/dS).
Transitions used to identify 14 different GLS are presented in the Additional file 4. Individual GLS were quantified using single ion monitoring and the data obtained are presented as μmol/g dry weight of sinigrin equivalents.
Levels of individual lipids were quantified using spiked internal standards.
PLA signals represented by individual punctas were quantified using Omero.
Transfection performances of individual conditions were quantified using microscopy-based image cytometry.
Red and green fluorescence intensity, respectively, in the individual cells were quantified using FV10i software (Olympus).
Transcripts of individual genes were quantified using primers specifically designed based on their gene sequences.
The individual signals were quantified using the optical system software programme ArrayVision, version 8.0 (Imaging Research Inc).
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