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Prior to analysis, feathers were washed in a 2 1 chloroform:methanol solution for 24 hours, and air-dried for a further 24 hours to remove remaining solvent. 1 ± 0.2 mg of tissue from each individual was analyzed using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20 20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK).
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The 385K data from Swiss individuals was analyzed using the wuHMM algorithm [12].
Leaf morphology in 'Malmaison' and 'St Anne's' individuals was analyzed using the AAM Toolbox [18].
The spontaneous swallowing of saliva of 111 healthy individuals was analyzed using surface electromyography (SEMG) and a computerized kinesiography of mandibular movement.
Genetic divergence among four pairs of haplotypes of Ma .balthica individuals was analyzed using the ape v.3.0-8 [ 39] and seqinr v.3.0-7 [ 40] R packages [ 41].
Absolute reliability, the degree to which repeated measurements vary for individuals, was analyzed using the coefficients of variation (CV), standard errors of measurement (SEM), and Bland-Altman plots (95% LOA) [ 31].
T cell responses from 16 HLA-B*35+ HIV-1 infected individuals were analyzed using the IFN-γ enzyme-linked immunospot (ELISpot) assay over the first years of infection.
These individuals were analyzed using RAD sequencing [ 11, 21] in order to identify SNPs.
The SMA-related genes of 212 Chinese healthy individuals were analyzed using MLPA assays.
A total of 470 admixed individuals were analyzed using SPAMIX (see Table 1 and Figure 7).
Nineteen sub-populations (586 individuals) were analyzed using 19 neutral markers.
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