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The simulated population pool consists of approximately 300,000 individual "samples", with each sample representing 1,000 individuals.
COD removals and coulombic efficiencies corresponded well between the two reactor designs for individual samples, with 66 92% of COD removed for all samples.
The fourth step would involve verification of the previous results using independent individual samples with the same disease.
Immunoglobulin G was prepared from pooled and individual samples with Melon G resin (Thermo Scientific) and checked for purity with denaturing polyacrylamide gel electrophoresis and Coomassie Blue staining.
Genotypes were quality controlled with the following criteria: individual samples with genotype call rate <90% and markers with call rate <96% or Hardy Weinberg P-value <0.001 were excluded from analysis (see supplementary material –SOM, for summary of locations, markers tested and analysed).
These calls were filtered according to log Bayes factor (posterior measure of confidence in the call) and proximity to the centromere and CNV calls from individual samples with an extreme number of CNV calls across their genome were also excluded (see Methods).
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Technical triplicates were performed for each individual sample, with negative controls in duplicate.
In dot plot graphs, each dot indicates an individual sample, with results expressed as median with interquartile range.
Each reaction was conducted in triplicate on each individual sample with ACTB and 18S rRNA amplified as internal control genes.
In order to achieve high selectivity and sensitivity, each channel contains an individual sample with pretreatment and detection zones.
We further tested if the correlation of an individual sample with a specific subtype was significantly greater than zero (Pearson's product moment correlation test).
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