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Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains.
Selected fosmids were then 'fingerprinted' through individual restriction digestion with EcoRI, HindIII and BamHI (4 h at 37°C) (Promega, Southampton, UK) before running 10 µl of each digest in a 1% (w/v) agarose/TAE electrophoresis gel for 12 hrs at 0.5 V cm−1.
For Southern blot, 10 μg of purified genomic DNA was digested with individual restriction enzymes.
Figure 3 indicates that most of this patterning results from the effects of the individual restriction enzymes.
The effects of individual restriction enzymes on cDNA-pool coverage, based on all 92 species (Table 3; see additional file 1).
The chosen form of therapy should allow the best possible rehabilitation, that is, a low risk of local failure and a life free of pain or individual restriction.
Similar(42)
He proposed a fundamental redesign of existing plant regulations, ending a jumble of individual restrictions on various compounds.
After more than a decade of growing tensions with federal regulators, fishermen here and throughout New England are pushing for a new system based on group quotas that would avoid these individual restrictions.
The aim of this paper was to address whether regional opportunity structures have an impact on educational attainment net of institutional and individual restrictions and how opportunity structures influence pupils' educational attainment.
Alas, because of states' individual restrictions on products with CBD and THC in them, the elixirs are currently only available in California (for about $7 a bottle and $35 for a sampler box of 5).
Optical mapping is unique among methods for analyzing genomes in that large-scale organizational information about the genome is preserved by physical attachment of large DNA fragments to a surface and assembly of a restriction digestion map based on imaging of a large number of individual restriction-digested genomic DNA molecules bound to the surface [ 4, 5].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com