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Such PKSs contain a single set of iteratively used individual proteins for the construction of a highly labile poly-β-carbonyl intermediate that is cyclized by associated enzymes to the core aromatic polyketide.
On the one hand, this allows for making full use of the vast amount of detailed biological knowledge about the molecular processes in and at the individual proteins for the setup of the computational model.
As theoretical proteome studies are not weighted by the copy number of the individual proteins, for lack of relevant data related to the quantity of individual proteins for the entire proteome, these observations are impossible to make.
An expert-driven functional selection approach was also performed to select individual proteins for comparison to our automated approach.
Detailed structural data for allergens will aid in designing individual proteins for SIT that have reduced allergenicity while retaining the ability to generate a protective immune response.
Microsoft Excel was used to generate plots of the number of hits from 3D7 proteinsrofeins of the different Ups types along the length of individual proteins for both IT4 and 3D7 proteinsroteins.
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This data should provide a valuable resource for the field and allow specific hypotheses to be generated for individual proteins of interest, particularly for those with no known function.
The relationship between protein detection and mRNA expression was explored both globally and for individual proteins of interest.
To accommodate multiple hypotheses testing issues, the significance for individual proteins or for biologic pathways, is based on a 5% FDR criterion.
However, ubiquitin also has important roles in modifying the function of individual proteins required for specific processes, e.g. ubiquitination of PCNA (proliferating cell nuclear antigen) is required for bypass of replication blocking lesions in DNA [6], [7], [8].
Because O. carboxidovorans growth was different under different conditions (see Results and Discussion), the ΣXcorr was normalized to account for differences incurred by different growth: the ΣXcorr (for the individual protein)/ΣXcorr (for all identified proteins in a dataset) was calculated.
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