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After the feeding trials, remaining leaf material of individual leaflets was weighted and analysed for HCNp.
To exclude unknown variability of HCNp from the experimental setup, we measured cyanide in remaining individual leaflets after they had been used in feeding trials.
As early instars, they mine individual leaflets, while later instars tie leaflets together into silken feeding tubes; individual plants (up to 40 cm diameter and 10 cm tall) may host dozens of larvae.
Bifoliolate leaves inferred from the symmetry of individual leaflets.
Conversely some may feel this was unfair as individual leaflets were taken out of context and consequently did not cover all the aspects of care we evaluated, for example, if there was a separate leaflet issued from physiotherapy, mobility and stairs may not have been covered; equally complications after surgery could have been covered elsewhere.
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Regular solution theory on the mean-field level is used to describe the free energy in each individual leaflet.
Thickness changes in membranes can be caused either by conformational changes in individual lipids tilting or folding up, reducing individual leaflet thickness, or by increased interdigitation of the lipids in each leaflet allowing closer apposition of the leaflets.
The tomogram is not sharp enough to distinguish individual membrane leaflets, thus it is not possible to discern whether the complex exists as hemifused stalk-like structure or possibly as a constrained pore with joined lumens.
From the viewpoint of the interactions with chemicals, monolayer systems differ from the bilayer, inter alia, by the absence of the opposite headgroup region that leads to a faster achievement of the equilibrium due to the removal of the potentially slowest step, the flip-flop of amphiphilic molecules between individual bilayer leaflets (section ).
The putative membrane breaches are observed not only at higher microscope defocus settings (e.g. 5 μm in Figure 5B), but also closer to true focus as well (3 μm in Figure 6) where fringing is less pronounced and where individual liposomal leaflets (still associated as a bilayer) can be observed lining the channel through the liposome's pinched neck (see Figure 6A slice 1, and Figure 6B).
Individuals were considered to be homozygous for all loci as the DNA preps were from a single leaflet of a single inbred accession.
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