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This allows us to quantify the kinetics and characteristics of individual fusion events from the perspective of the lipids or the DNA-lipids involved in the process.
This provides a significant increase in the number of cells that can be acquired, but sacrifices the ability to resolve individual fusion events, while maintaining the highly resolved sub-cellular quantification (Lovy et al. 2012).
Furthermore, we can note that individual fusion algorithms do not appear to produce consistent threshold values over conditions (i.e., we do not see clear vertically striped color patterns in the heatmap).
When mitochondrial fusion rate was quantified as the number of individual fusion events per mitochondria per min, there was 55.3% ± 3.5% decrease in the fusion rate in Trak1-depleted cells compared to the control cells (Fig. 2D).
Individual fusion reactions are executed by distinct combinations of SNARE and SM proteins to ensure specificity, and are controlled by regulators that embed the SM-SNARE fusion machinery into a physiological context.
At the gel front of the first dimension, individual fusion proteins together with degradation products were detected.
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We found that oligomerization of aSyn increased the half life of each of the individual fusions by ∼40%.
The exocytosis of individual fusion-competent vesicles is mediated by the stochastic gating of one or few CaV1.3 channels located within a few nanometer from the release site and such "nanodomain control" of neurotransmitter has been proposed to permit temporally precise synaptic coding even for weak stimuli [ 103].
Considering that individual fusions may be observed relatively rarely (even uniquely), the potential functional consequences of gene fusions assumes priority over considerations of recurrence.
When β-galactosidase activities for the individual fusions were compared between Rt2472 and Rt24.2, significant differences in the levels of transcription were found for crp1, pssY, rapA1, gelA, prsD, ndvA, celA (Fig. 5a), flaA, and rem genes (Fig. 5b).
Interestingly, the percentage of transgene-specific clones generated in the three individual fusions correlated with the malaria antigen expression level of the corresponding transfected cells used for the immunisation of mice.
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