H9 cell nuclear extract was fractionated, and individual fractions were analyzed for TAFs by immunoblotting.
Even without glycoprotein enrichment but with some fractionation before MS analysis of individual fractions, we have identified a large number of mucins in plasma studies (Table 1).
Dried powder of stem bark of C. griffithii was exhaustively extracted serially by soaking in petroleum ether, acetone and methanol to fractionate the chemical constituents into individual fractions or extracts.
To investigate whether the six hESC TAFs were associated in a stable complex, H9 cell nuclear extract was fractionated by sucrose gradient sedimentation and individual fractions analyzed for TAFs 2, 3, 5, 6, 7 and 11 by immunoblotting.
A feature of the IPAS platform is that extensive fractionation allows de-complexing of the samples into individual fractions to allow identification and quantification of proteins present in the plasma over 6 7 orders of magnitude [16], [19].
HeLa cell nuclear extracts were size-fractionated by gel filtration, and the presence of SIRT6 in individual fractions assessed by Western analysis.
Individual fractions were analyzed by microcapillary electrophoresis (Agilent Bioanalyzer, GE Healthcare).
The approach developed assumes that the material resistance to cavitation may be described satisfactorily by its response to individual fractions of cavitation pulses amplitude distribution.
The backbone of the approach is a differential superposition law, which allows modelling erosion progress under polyfractional cavitation load by keeping continuous control over erosive process inputs contributed by individual fractions of cavitation pulses distribution.
Individual fractions were characterised for (1) starch, aNDF, and water content, (2) water absorption index (WAI) and water solubility index (WSI), (3) viscosity profile during cooking and cooling in excess water, and (4) in vitro starch digestibility.
High affinity H2 oxidation activity measured in individual fractions was proportional to the copy number of hhyL gene, specifying the large subunit of putative high affinity [NiFe]-hydrogenases. 2.5% of the hydrogenase activity was recovered in bacteria-free soil extract.
