Sentence examples for individual cloning from inspiring English sources
For each individual, cloning stages must be added so that some 10,000 extra bp DNA had to be sequenced per individual, or about 390,000 bp total to obtain reliable sequences for just four loci sequenced in every individual.
The individual cloning success as well as the degree of cloning at the stand level are distinctly higher in the CWS (N/ G is doubled and C is even seven times larger).
Particularly in the CWS, individual cloning success via root suckering was observed to exist at distinctly higher degrees compared to our investigations in a present-day HFS (group selection shelterwood system) which is believed to represent (near-) natural forest reproduction patterns.
The quantity of clones of individual cloning was count, and then 10 clones from individual cloning were randomly picked and regular colony PCR with correspondent primer set was conducted to confirm positive clones.
Current technology for screening the TCR repertoire for expanded T-cell clones relies on 'spectratyping' [4], often referred to as immunoscope, and/or individual cloning and sequencing of a sample of the T-cell population [2], [5] [9]].
Unique cloning sites were also included between each in-frame MAP ORF and at each end of the construct to facilitate cloning of each ORF into individual cloning vectors (pQE80 series, Qiagen, UK).
Horizontal rows represent individual cloned alleles.
Genomic DNA isolated from individual clones was examined by PCR.
In parallel, the PCR products were cloned and a total of 105 individual clones were sequenced.
Four samples with three to five genotypes detected were cloned and individual clones were sequenced.
The cytogenetic properties of individual clones were highly heterogeneous and chromosome instability was observed in both spontaneous and radiation-induced mutants.
