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To develop a method for characterizing the transcriptome of individual cell types in the inner ear sensory epithelia.
Subsequently, we correlated gene transfer efficiency with cellular uptake, pH responsiveness of nanocomposites, and phagosomal pH of individual cell types.
In PBMC cultures, individual cell types were distinguished from one another based on differential antibody staining and on FS and SSC properties.
Obviously, organ culture of the whole trigeminal ganglion or of the individual cell types, neuron or glial cells, are not models of any primary headache type.
The growth and differentiation of individual cell types was determined using live cell fluorescent microscopy demonstrating the utility of fluorescent labels to monitor tissue organization in real time.
Although the brain regions involved in regulating addiction processes have long been identified, the ways in which individual cell types govern addiction behaviors remain elusive.
Interrogation of gene regulatory circuits in complex organisms requires precise tools for the selection of individual cell types and robust methods for biochemical profiling of target proteins.
In addition, it is impossible to attribute observed differential expression to individual cell types.
A comprehensive cellular analysis will be important to link the differential expression of specific subunits to individual cell types.
CPMV was also detected in spleen and lymph nodes, however the individual cell types that interacted with virus were not determined [26].
The repertoire of AP-1 dimer composition allows tailoring of an AP-1-mediated response by individual cell types to a given stimulus.
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