c, Average Pearson's correlation of replicate sgRNA counts (n = 86,875) for individual cell lines.
Detergent lysis of tissue culture cells provides a simple fractionation procedure that can be optimized to individual cell lines.
This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines.
The studies that claim different fermented products have anticancer properties, or the ability to control cholesterol and hypertension, have mostly been carried out on laboratory animals or individual cell lines.
The ability to accumulate multidimensional data pertaining to the same individual cell lines, from complete genomic sequences to detailed gene regulatory profiles, further enhances the utility of LCLs as a model system.
Individual cell lines, each expressing a different FcgammaR, bound human IgG, as evidence that the physical properties of the receptors were preserved.
The individual cell lines were derived by cultivation with the increasing cisplatin concentration in cultivation medium.
Four mutations were identified in four individual cell lines: OVCAR 10, OV90, Hey and ES-2 (Figure 1).
The individual cell lines were maintained in Opti-MEM media supplemented with 10% FBS and 4 µg/mL puromycin.
Total RNA was extracted from individual cell lines using RNeasy Mini Kit (Qiagen, Valencia, CA) and quantified using a NanoDrop 1000.
Individual cell lines contributed to various degrees to the fraction of cell-specific MP genes, with HUVEC and G361 contributing most.
