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The LEM-based interpolation framework presented in this work was developed to fill in the existing gaps within individual cell line response data as a function of NP concentration under photon irradiation.
This developed framework is intended to fill in the existing gaps of individual cell line response as a function of NP concentration under photon irradiation and assist the scientific community in planning future pre-clinical trials of high Z nanoparticle-enhanced photon radiotherapy.
This LEM-based framework was developed to fill in the existing gaps of individual cell line response as a function of NP concentration under photon irradiation to assist the scientific community in planning future pre-clinical trials of high Z nanoparticle-enhanced photon radiotherapy.
For every individual cell line of interest, equimolar amounts of all purified PCR fragments were pooled.
Expression profiling data from 1936 individual tissue samples and 929 individual cell line preparations were evaluated in this manner.
Growth studies with each individual cell line were done at least three times and similar results were obtained consistently.
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Detergent lysis of tissue culture cells provides a simple fractionation procedure that can be optimized to individual cell lines.
The individual cell lines were derived by cultivation with the increasing cisplatin concentration in cultivation medium.
Total RNA was extracted from individual cell lines using RNeasy Mini Kit (Qiagen, Valencia, CA) and quantified using a NanoDrop 1000.
Four mutations were identified in four individual cell lines: OVCAR 10, OV90, Hey and ES-2 (Figure 1).
The individual cell lines were maintained in Opti-MEM media supplemented with 10% FBS and 4 µg/mL puromycin.
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