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The calculated free energy landscapes of individual backbone and sugar degrees of freedom expectedly show the greatest change in the vicinity of the flipping base itself, but specific delocalized effects can be discerned upto four nucleotide positions away in both 5' and 3' directions.
Here the crystal structure of the RrgBD2 D4 backbone subunit fitted into the EM reconstruction of the S. pneumoniae pilus reveals for the first time the polymeric architecture of a Gram-positive pilus indicating a head-to-tail organization of individual backbone subunits.
Positional variance was computed by summing the deviation of individual backbone atom position and dividing by the number of backbone atoms in the loop.
Amide protection at an individual backbone amide group was determined as where P(R i ) is the protection of a backbone amide at residue i.
To circumvent this problem, we introduce an online chemical reduction step following completion and quenching of the HDX reactions and prior to the top-down MS measurements of deuterium occupancy of individual backbone amides.
A more intuitive presentation of these data shows protection levels of individual backbone amides, which were calculated for each residue by subtracting the deuterium content of adjacent fragments in either the c- or z-ladder.
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To show this, we iteratively reassemble the helix bundle of each protein using only these interactions, generic interaction geometries, and individual helix backbone conformations.
Bottom: individual IST1 backbone amide resonances perturbed (1) or not (−1) upon addition of ULK3 MIT 2.
Thus, this phase is a hexagonal columnar mesophase (Colh) formed by the parallel alignment of the individual polymer backbones separated from each other by a continuum of molten aliphatic chains.
Besides the difference in reactivity between RA patients (especially the anti-CCP2-positive patients) and the healthy controls, a difference also was found in the size of signals against the individual peptide backbones that was common to both citrullinated peptides and to arginine-containing control peptides, as well as to both RA patients and controls.
Four individual isomers of backbone re-organized ezetimibe analogs were designed and synthesized.
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