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The unmapped data and all assembled data from each individual assembly were combined and then re-assembled using CAP3 to generate a final consensus assembly for the further identification of the AS events.
Over 70% of reads were fully assembled, and close to a further 10% of reads were partly assembled in both the assembly of all reads together and in each individual assembly run (Table 1).
In an internal pilot project, a team of robots is working autonomously on individual assembly steps.
Each product is derived from individual pieces of material, individual components, and individual assembly processes.
These nanostructures are manipulated using dielectrophoretic forces, allowing their individual assembly and characterization.
The conventional approaches to decreasing WMSDs in assembly workers usually focus on individual assembly work at the station level.
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Assembly 13 was a merger of the individual assemblies, while Assembly 10 was one of the all-in-one assemblies with different k-mer lengths.
Singleton reads in the individual assemblies, when assembled together, created new contigs, isotigs and isogroups.
Trinity outperformed Trans-ABySS in assembly continuity (Table 5), and for both assemblers, merging individual assemblies decreased contig length, particularly for Trans-ABySS.
The contigs resulting from the individual assemblies were then assembled together with the original Illumina reads using a k-mer length of 67 with the conserveLong option turned on.
After removing duplications using this strict standard, for the Trinity_AFSFASSS that was assembled without merging individual assemblies, 27.52% of its contig number and 35.87% of its total length had been reduced.
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