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Increased accuracy in sample representation, and allele frequency estimation may have been achieved by performing separate PCR reactions in each individual and pooling of PCR products [22], although this would have resulted in increased costs and DNA use.
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Individual and pooled sera from both rabbits immunized with each extract were kept at −20°C.
Individual and pooled OR estimates derived from a random effect model analysis were illustrated in the Forest plot.
Individual and pooled OR estimates derived from a random effect model analysis have been illustrated in a forest plot (figure 2).
With sample mix-up control and enrichment cost reduction in mind, we evaluated the enrichment of barcoded SOLiD libraries (individual and pooled), rather than preparing NGS libraries after enrichment [ 8- 10].
Rather than testing individual cytosines and pooling them into putative DMRs, MD considers changes in the methylation profile's shape over a given region.
Hemolymph was collected from the adductor muscle of 5 naïve individuals and pooled into 4 pools (2.5 ml of hemolymph per pool).
Cuticular extracts were collected from these individuals and pooled according to treatment.
For the characterization of mitochondrial and nuclear markers we analysed individuals while the screening for Wolbachia included extracts from both, individuals and pooled individuals.
Moreover, this discrepancy increased in the 26-week-old females (hmmr m/m n = 2, n = 2, n = 9 collected from 3 individuals and pooled; hmmr +/+ n = 17).
Such expanded transcriptome size is usually evidence of isoforms of the same gene and artifacts such as sequencing errors, repeats, and variations in coverage and genetics within diploid individuals and pooled samples [ 24].
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