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Individual affinity column elutions were collected, pooled and confirmed by SDS-PAGE, then aliquoted/frozen at −80 °C.
The individual affinity of each lipid for SUMO-2 in the mixture was calculated from a global analysis of the combined data (Fig. 3b).
Similar(58)
We also used the maximum likelihood (ML) algorithm implemented in the frappe software [24] (http://smstaging.stanford.edu/tanglab/software/frappe.html) to estimate individual affinities for a pre-determined number of clusters.
Protein DNA interaction analyses necessarily measure a relatively large number of individual affinities.
Firstly, I have a concern about the novelty of their theoretical advance (apparent affinity can exceed individual affinities): is ultra-affinity related to kinetic proofreading?
The second approach was more qualitative and used titration of CR species into RAP followed using the same fluorescence perturbation technique as used for determining individual affinities above.
While one would be tempted to assume that the affinity of a multi-RRM domain protein would be the product of the individual affinities, this turns out to be incorrect.
Proof of principle was demonstrated by separating a mixture of five compounds known to be estrogenic (17β-estradiol, 17α-ethinylestradiol and the phytoestrogens coumestrol, coumarol and zearalenone), followed by post-column bioaffinity screening of the individual affinities for ERα.
It is likely that 18 F-DPA-714 is also sensitive to this variable inter-individual affinity state.
This precluded also any potential effect of inter-individual affinity [8].
For this example you want to test the correlation (if any) between an individuals affinity for cats and their overall happiness.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com