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Electrolytes perform as indispensable reaction medium during electrochemical processes, which can be further classified into solid oxide electrolytes, molten salt electrolytes, polymer electrolytes, and liquid electrolytes.
An otherwise dispensable reaction appears to be indispensable if the enzyme that catalyzes this reaction is required to be present in the network, owing to its pleiotropic function in catalyzing an indispensable reaction.
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We additionally conducted knock-out simulations, which revealed a set of indispensable reactions, representing putative drug targets.
To this end, we distinguish between indispensable reactions, which make up most of the biomass production and dispensable reactions, which have a negligible contributions to growth.
A nonactive reaction can be stably retained in the network if the enzyme that catalyzes it also catalyzes one or more indispensable reactions.
To address the first aspect, we repeat the network minimization for three different thresholds to distinguish between dispensable and indispensable reactions.
The results of the functional enrichment analysis and biological interpretation of the metabolic role of indispensable reactions, showing flat profiles of fractional appearance, supports our assumption that these reactions constitute the most crucial part of the metabolic network.
To be conservative, we also used a more stringent cutoff of f < 0.9 in defining indispensable reactions, for which the error rate is expected to be 0.6% (3/486) for FBA and 1.2% (6/486) for MOMA, respectively.
Such indispensable redundant reactions are referred to as efficient reactions, as they are more efficient than other reactions of the same functions under at least one condition.
Knock-out simulations identified 307 indispensable metabolic reactions within the parasite.
These observations suggest that the estimation of the number of indispensable redundant reactions is relatively accurate (see Materials and Methods).
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