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Unlike the normal RNA sequencing library preparation step, disposing samples through methods such as ribosomal RNA depletion and poly (A) minus RNA selection alone or in combination with RNase R treatment is indispensable prior to library construction, which has favored the discovery of circRNAs (Danan et al., 2012; Jeck et al., 2013; Zhang et al., 2013).
However, the enzyme inhibitors in pretreatment hydrolysate make solid substrate washing and hydrolysate detoxification indispensable prior to enzymatic hydrolysis.
A better understanding of the molecular characteristics of the different ovarian cancer subtypes and their specific molecular signatures is indispensable prior to development of new screening strategies.
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However, removal of residual monomer M0 was an indispensable step prior to postpolymerization modification since incomplete monomer conversion was observed in the previous step, synthesis of polymer P0 using ROMP of maleimide-containing monomer M0.
In a Facebook post yesterday morning he wrote: The EIA is key and indispensable for the prior consultation processes [supposed to be held with potentially affected indigenous peoples] because it is the only way they have to evaluate whether they are for or against a project.
Although actual experiments used ex vivo corneas, the results found are indispensable to establish background knowledge, prior to an in vivo examination approach.
Results suggest that domain-specific prior knowledge is not an indispensable prerequisite to contribute 'somehow' to classroom dialogue.
To summarise, our analyses suggest that domain-specific prior knowledge is not an indispensable prerequisite to 'somehow' engage in classroom dialogue.
Thus, quality management in peer teaching with thorough and structured training of tutors prior to teaching activity is indispensable.
We would expect transfer of an indispensable gene to the nucleus to occur prior to loss from the organellar genome, followed by the acquisition of regulatory signals for the gene and plastid import signals for the protein.
For biochemical analyses, the application of strict conditions for sample preservation prior to collagen extraction is indispensable, as the stability of the hydroxyproline content strongly depends on the sampling procedure [ 28].
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CEO of Professional Science Editing for Scientists @ prosciediting.com