Programming and indexing tags with their corresponding video clips may be time consuming, as it is for any ultrasound video loop editing for educational purposes.
Designers can find suitable cases by selecting of index tags and the calculation of similarity with the existed cases in database to get related biomimetic keywords from TRIZ innovative principles.
Samples were tracked using "index tags" incorporated into the adapters.
All 60 samples were labeled using 60 different index tags (Nextera, Epicentre Biotechnologies).
After sequencing the index tags and libraries separately, the tag sequence information was used for assigning reads to the individual samples (8 ).
Multiplexing uses indexing tags, "barcodes" or short (≤ 20 bp) stretches of sequence that are ligated to the start of sample sequence fragments during the library preparation step.
Unique index tags of six bases provided in the Multiplexing Sample Preparation Oligonucleotide Kit were added in the PCR step to discriminate between the 16 samples.
After purification, the samples with different index tags were pooled together (2 μl each) and concentrated with DNA Clean & Concentrator-5 (Zymo Research).
Illumina adapters containing indexing tags were ligated to the ends of these 3′-adenylated cDNA fragments followed by two purification steps using Agencourt AMPure® XP beads (Beckman Coulter).
Each enriched library (having six samples with different index tags) was sequenced in a single lane of an Illumina HiSequation (100 bp, multiplexed, paired end for chr5; 100 bp, multiplexed, single end for chr1 and chr2).
Finally we developed a custom set of index tags and primers which increase the number of samples that can simultaneously be sequenced on a single lane of an Illumina instrument.
