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To compare transcriptomic responses between datasets, two related indicators of transcript variance were estimated per transcript per dataset, the variance mean ratio (VMR) and the coefficient-of-variance (CV) [35], [36].
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Additionally, the number of exons can be used as an indicator of transcript quality.
The analysis of the relative abundance of individual ESTs that make up unique sequences (contigs) from different libraries can be used as a first indicator of transcript abundance.
An accepted method of identifying large scale differences in gene expression is to use EST abundance as an indicator of transcript abundance.
For the determination of the global A. gambiae female salivary gland transcriptome, the microarray spot hybridization fluorescence intensities were used as an indicator of transcript abundance/expression level, since each spot contained a similar amount of nucleic acid.
d P value of a t test as an indicator of significant transcript variation in responders versus non-responders.
cSignificance analysis of microarrays (SAM) value as an indicator of significant transcript variation in responders versus non-responders.
We used the median contig length (N50) generated for each k-mer as an indicator of the transcript contiguity of de novo assembly.
These results suggest that ORF length, not the sequence length, is a better indicator of finding transcripts with protein coding capacity and subsequently getting a hit in sequence databases.
These results suggest that ORF length, not sequence length, is a better indicator of finding transcripts with protein coding capacity and subsequently getting a hit in a sequence database.
Table 2 shows success and error rates of variety of indicators of the resulting dog transcript annotation.
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