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In model-based meta-analysis, linear models are used to combine indicators of expression patterns from individual studies (e.g. fold changes or differences between mean groups, standardized estimates, or normalized values) and associated test-statistics or functions used to evaluate the expression pattern across studies.
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In light of this point, we selected a TCS as an indicator of expression profile of 89K.
To do this, we used the number of ESTs/cDNAs as a broad indicator of expression level.
In contrast, the propensity of promoter DNA methylation is a stronger indicator of expression breadths than of expression levels.
While exon size, intron length, CAI and d N are stronger determinants of expression levels, CpG O/E of promoter is a better indicator of expression breadths.
However, this does not correlate with expression in adult tissues [ 40] and imprinted differential methylation is not a good indicator of expression levels in human adult tissue.
All proteins identified by Task4 were correlated based on their standardized gene expression score, i.e. the ratios between the individual expression levels divided by the average of all expression levels as a relative indicator of expression (see Methods).
Variance is an indicator of expression noise, and genes with larger amounts of expression noise have been shown to be less important in organismal survival and functionality [ 30- 32].
The next best approach is to have the indicator of expression and the effector inserted at the same chromosomal location, which we were able to achieve for all cases except the assays of the 2 hr aversive and appetitive odor memory which used a weaker UAS-Shibire effector based on a P-element insertion.
The presence of wild-type p53 and occurrence of apoptosis may be important indicators of MT expression, since the expression and induction of MT has been associated with protection against oxidative stress and apoptosis.
Additionally, besides expression abundance as an indicator of gene expression behavior, expression breadth and specificity are also worth investigating [6], [7], [33].
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