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Total protein assays and western blot analysis of osteocalcin expression were used as indicators of cell proliferation and differentiation.
The researchers detected more indicators of cell repair and inflammation in the post-workout samples than in the pre-workout samples.
Cells were loaded with Calcein-AM or tetramethylrhodamine methyl ester (TMRM), two probes employed as indicators of cell viability/morphology and mitochondrial membrane potential, respectively.
Finally we chose to evaluate three indicators of cell function: expression of a key transcription factor, expression of selected transcripts, and assessment of cell morphology.
These correlations revealed that structural features imparted by the Pluronic® F127 micelles were able to reverse the normally strong correlations found between indicators of cell spreading and the hydrogel's mechanical properties.
Quantitative phase signals have recently been used to derive biological indicators for live cell observation, such as the measurement of dry mass [13], cell differentiation based on morphology [14], or early indicators of cell death [15], to cite some possible applications.
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Our results point to changes in regulatory DNA landscapes as quantitative indicators of cell-fate transitions, lineage relationships, and dysfunction.
But which factors are the most important indicators of cell-type specific TF binding and to what extent do combinations of factors determine cell-type specific binding?
Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell "rejuvenation".
It uses resazurin as color dye to measure the metabolic capacity of cells - an indicator of cell viability.
The bactericidal activity was examined with 2, 3, 5-triphenyl tetrazolium chloride (TTC), an indicator of cell viability.
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