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In contrast, nicotine did not affect colony density (Figure 2B, left panel) or the number of cells per colony (Figure 2B, right panel) which are indicators for cell proliferation.
Here we present a systematic comparison between cultivation-based assessment of cell viability and cultivation-independent indicators for cell viability.
This study contains a systematic comparison between cultivation-based assessment of cell viability and cultivation-independent indicators for cell viability by sampling and analyzing heat-exposed B. subtilis cells going through the transition from culturable to unculturable cells.
However, the feasibility of cultivation-independent biomarkers under a number of well-defined conditions as indirect indicators for cell viability is still a matter of debate [ 1, 3, 4].
We chose to use the tomato polygalacturonase 1 (Tand1) and 4 (TApromotersoters ligated to a GUS reporter gene as indicators for cell separation in tomato (Hong et al. 2000).
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Besides as a mesenchymal marker, vimentin is a novel indicator for cell proliferative activity and undifferentiated status in liver cells.
Frozen sections of carotid arteries were stained with the kit to assess DNA fragmentation as an indicator for cell apoptosis.
Therefore, the level of 16S rRNA is not a very good indicator for cell viability under these conditions.
The ratio of PI3K activity in rIGF-1 treated cells and vehicle treated cells were used as an indicator for cell sensitivity to IGF-1.
To measure DNA synthesis as indicator for cell division 25 μg/ml BrdU (from Sigma-Aldrich) was added to the cell culture for 1 h.
MDA is a metabolite that is produced during lipid peroxidation of cell membrane and is used as an indicator for cell damage [ 56].
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