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A purple (C. violaceum CV026) or blue (A. tumefaciens NT1) pigment produced by the indicator strains is indicative of quorum sensing activity of the tested strains.
Indicator strains were cultured on the Columbia blood agar plates.
The antimicrobial peptides production was measured as zone of inhibition against indicator strains.
Cell suspensions of indicator strains were obtained by adjusting OD600 to 0.8 using sterile LB broth.
All Vibrio and Aeromonas species were used as target strains (indicator strains).
Activity spectra (100 μg/ml) were determined with the selected indicator strains as shown in Table 1.
However, all growth media encouraging the growth of the indicator strains were previously detailed described (Embaby et al. 2014a).
All indicator strains including human and plant pathogens along with their specifications used in this study were previously detailed mentioned (Embaby et al. 2014a).
Peaks were collected and analysed for their potential bactericidal effect by the spot-on-lawn method with S. aureus CICC 10384 as indicator strains (Yue et al. 2013).
Indicator strains were inoculated in LB broth, incubated 24 h at 30 °C with shaking (150 rpm), and optical density at 600 nm (OD600) was determined.
Six test strains stabbed uniformly across the agar surface of 9-mm Petri dish completely inhibited the growth of indicator strains.
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