Sentence examples for indicator reaction from inspiring English sources

The detection of mechanistic changes hinges on linking fluorescence reporters to selected reaction components, either directly (chemically) or indirectly (via an indicator reaction).

The HRP-catalyzed oxidation of TMB with hydrogen peroxide was used as an indicator reaction.

This latter advantage is important because it eliminates false signals based on a label or indicator reaction and avoids the time, expense, and expertise needed to modify target compounds for optical assay.

The indicator reaction (0.5 ml total volume) was performed at 37°C in 100 mM HEPES/KOH (pH 7.5, RT), 10 mM MgCl2, 5 mM phosphoenol-pyruvate, 0.5 mM NADH, 3.5 units of pyruvate kinase and 2.5 units of lactate dehydrogenase.

The indicator reaction (0.5 ml total volume) was performed at 37°C in 100 mM HEPES/KOH (pH 7.5, RT), 10 mM MgCl2, 5 mM ADP, 0.5 mM NADH, 3.5 units of pyruvate kinase, 2.5 units of lactate dehydrogenase and 100 μl aliquots from the glycerate kinase assay.

Based on the literature survey only a few numbers of indicator reactions for the kinetic determination of TU by spectrophotometric method have been published (Abbasi et al. 2009; Abbasi et al. 2010).

From various studies using indicator reactions, it was shown that over a wide range of kingdoms, the ratio between cytosolic (NAD + /NADH) and (NADP + /NADPH) are in the order of 100 (Bautista et al. 1979; Satrustegui et al. 1983; Greenbaum et al. 1971; Canelas et al. 2008).

All of these methods are based on the catalytic effect of the TU on the certain indicators reactions.

Mass transfer coefficients were determined using the limiting current technique, with the cathodic reduction of potassium ferricyanide ion in excess of potassium carbonate as the indicator electrode reaction.

In this contribution we suggest the feasibility to use the NMR transverse relaxation rate, R2, as an indicator of reaction evolution during the heterogeneous catalytic hydrogen peroxide decomposition: 2H2O2 liquid)→2H2O2 liquid.

For example, calculation of E-factor (or other mass-based indicators) across reaction sequences built from Reaxys to allow their scoring, returning a greater amount of information to the user, would be possible, as shown in [9].