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Phospho-Histone (H3) is an indicator of mitotic activity.
MPM-2 is commonly used as an indicator of mitotic disturbance.
MPM-2 is an antibody that recognises a group of proteins whose epitopes are exclusively phosphorylated during mitosis – particularly early prophase to metaphase (Davis et al, 1983) and it is commonly used as an indicator of mitotic disturbance.
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Typically, an imaging screen for mitotic phenotypes would be performed as a fixed endpoint assay in microplates with immunofluorescence staining for DNA and indicators of mitotic arrest and perhaps also apoptosis [20].
The M phase indicator mitotic index, the number of mitotic bodies in a microscopic field, and the S-phase fraction, a DNA flow cytometry determination, are used to measure the tumor proliferation rate and are predictive for breast cancer prognosis [ 2- 4].
Therefore, telomere length may serve as another good indicator for mitotic history and the prospective additional life span.
This can be as indicator of advanced disease as high mitotic activity.
Mitotic indices also demonstrated similar numbers of mitotic cells in control and chrysotile-treated cells.
The density of mitotic cells indicated by mitotic marker PH3 also significantly decreased in DKO mice.
The mitotic index was expressed as number of mitotic figures in 10 high-magnification fields.
The percentage of mitotic cells (mitotic index) was determined with DAPI staining.
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