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Towards this end we have defined a specific temperature during infection that is an ethical indicator of death (unpublished observation).
Interestingly, avicin D treatment also led to an elevated cleavage of Caspase-8, an important indicator of death receptor-induced apoptosis (Figure 1E).
While their automated screening assay was five-times faster than screening manually, the algorithm was limited to a simple yes-no (live-dead) assessment using the uptake of SYTOX® Orange as an indicator of death, and was unable to quantify the effects of compounds on individual animals.
If the number of dead patients is large, an indicator of death is desirable.
Time to positive culture result (which is correlated with the level of bacteremia) is a prognostic indicator of death.
Our results confirm the recent finding from Hadjizacharia and colleagues that CDI is an independent risk indicator of death [ 21].
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(Reflecting these concerns is a landmark 1968 report published by a Harvard Medical School committee led by physician Henry Beecher (Ad Hoc Committee of the Harvard Medical School 1968).) Soaring medical expenditures provoked concerns about prolonged, possibly futile treatment of patients who presented some but not all of the traditionally recognized indicators of death.
We also successfully managed to use three innovative proxy indicators of death in the absence of a formal and functional vital registration system.
This is also one of the first reports to use proxy indicators of death such as coffin sales and church funerals both of which show a declining trend over time.
Tachypnea, respiratory distress, deep breathing, shock, prostration, low pH, hyperparasitemia, severe anemia, and jaundice were statistically significant indicators of death in the univariate analysis but not in the multivariate model.
Overall, these results indicate that changes in ATP levels are a reliable indicator of cell death in stem cell populations upon drug insults and may have utility for hESC-based automatic screening assays.
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