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The second approach is to use the number of reads at any site as an indicator of copy number.
Copy number profiles can be inferred from sequencing data of sufficient depth and coverage, by using the relative number of reads mapped to a given genomic position (depth ratio) as an indicator of copy number.
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The differences in signal to noise and z-score between confirmed CNV bases and false positive CNV bases indicates that ratio is a stronger indicator of true copy number when signal variation is taken into account.
(5) shows the chipboard visible through the slats of the casing - there should be numbers and letters visible on the green board, however you may have a fake copy which says 'Nintendo' there instead- that is a certain indicator of a copy.
We used the fluorescent signal peak ratio as an indicator of the copy number ratio of the two alleles.
The PCR products were sequenced by an ABI3700 machine to compare the fluorescent signal peak ratio at the SNP locus as an indicator of the copy number ratio of the two alleles.
The number of distinct clean tags for each gene was calculated and then normalized to the number of transcripts per million clean tags (which is a standardized indicator of the transcript copies in every 1 million clean tags) [ 66, 67].
As described above, using a combination of DNA content and the spike factor of the sample, the GSC spike mRNAs become direct indicators of the copy numbers (C') per cell.
As FISH is considered to be a semi-quantitative technique [ 32], it is reasonable to expect that size and intensity of hybridization signals is an indicator of targeted sequence copy number.
To circumvent the influences of plasma DNA quantity and numerical chromosomal aberrations, we particularly calculated the ratio of the dosage of CCND1 to that of the reference gene, dopamine receptor D2 (DRD2) gene mapped to 11q22-23, an an indicator of the CCND1 copy number (CCND1/DRD2: C/D ratio) (Kyomoto et al, 1997).
Further assessment of RD and PEM as indicators for copy number may streamline minisatellite detection via WGS.
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