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Intracellular Ca2+ concentration was quantified by a fluorescent Calcium Green-1 Ca2+ indicator kit (C3010MP, Life Technologies, USA) according to the manufacturer's protocol.
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D'Alessandro", University of Palermo, Italy, using the mycobacterial growth indicator tube SIRE kit (Becton Dickinson, Sparks, MD, US).
Remove the strip from the water, and compare the color of the water to the chlorine test kit indicator guide.
One such study [ 78] has been performed with cultured neurons using an anionic probe, termed PMPI (plasma membrane potential indicator), from a commercial kit.
In multivariate Cox analysis, c Kit was an indicator of metastasis independent of 25 other concomitantly evaluated markers of prognosis.
Accumulation of glutamate in the culture medium was assayed by monitoring the reaction catalysed by glutamate dehydrogenase, using iodonitrotetrazolium chloride as a colorimetric indicator, all supplied as a kit (R-Biopharm).
Plasma membrane potential was measured in cultured neurons using a PMPI (plasma membrane potential indicator; a proprietary component of the kit) from the Molecular Devices membrane potential assay kit.
It will also be kitted out with an indicator or mechanism to highlight when it should be ditched for recycling and replaced with a new helmet.
Cells were treated with TRIzol (Life Technologies Corporation, New York, USA) to extract total RNA for real-time quantitative RT-PCR (qRT-PCR) of ECM indicators using the PrimeScript RT Reagent Kit (TaKaRa Inc., Dalian, China).
The level of c-Kit expression was an indicator of the differentiation potential of 10·5 dpc CD34+ AGM cells (Fig 1B).
Lactate dehydrogenase activity release, used as indicator of cell membrane damage, was measured by LDH assay kit (Cytotoxicity Detection Kit, Roche Diagnostics, Milan, Italy) on the culture medium of cells exposed to both MWCNTs for 24 h.
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