Finally, several important performance indicators, including illumination uniformity, system reproducibility and accuracy, are elaborately tested.
Mean brightness is an indicator of the illumination level.
Another problem encountered with fluorescent dyes is photodamage of the indicator under constant illumination generating less fluorescent species and a decreasing signal over time.
These genetically encoded reporters were first described by Hanson et al. (2004) as redox potential sensors with two fluorescence excitation maxima, thus permitting ratiometric analysis that minimizes errors associated with variations in indicator concentration (roGFP expression), illumination intensity, and cell thickness.
Light bars and circular illumination regime indicators show the timing of treatment and sampling in the day (Figures 4, 6 and 7).
DL, referring to a long-term light-emitting decay process after illumination, is a sensitive indicator of the functional states of many biological systems, including plants, animals, human cells and tissues [ 8, 9].
The self-report of seasonal changes in mood and behavior and of poor illumination indoors seem to be relevant indicators of the HRQoL and mental well-being.
The hands and the 12, 3, 6 and 9 indicators are all coated with Super-LumiNova to provide illumination in the dark, which does the job, though it does seem to have to spend a lot of time under direct sunlight to build up a decent and lasting charge.
Electroluminescent panels are of more interest as signal indicators and display devices than as a source of general illumination.
A comparative study has been performed with various calcium indicators under a continuous exposure to a 20 µW laser illumination, the energy close to the one used during confocal imaging.
