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Besides as a mesenchymal marker, vimentin is a novel indicator for cell proliferative activity and undifferentiated status in liver cells.
Therefore, the level of 16S rRNA is not a very good indicator for cell viability under these conditions.
Frozen sections of carotid arteries were stained with the kit to assess DNA fragmentation as an indicator for cell apoptosis.
We next employed a live imaging approach to examine the effects of docetaxel on chromatin condensation (an indicator for cell apoptosis).
Nuclear localization of Bcl-3 has been reported as an indicator for cell proliferation in different types of cancer [ 13, 15, 20].
One method of analysing the tumorigenic potential is the soft agar assay, which measures anchorage-independent growth, which is an indicator for cell transformation.
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The targeted DNA integration efficiency, as determined on the basis of the total number of HeLa cell clones analyzed was 24% (9/38), whereas if one considers exclusively clones with discernible changes in the AAVS1 locus (as a surrogate indicator for cells that originally contained enough Rep68) it reached 47% (9/19; Fig. 6B; Table 1).
In contrast, nicotine did not affect colony density (Figure 2B, left panel) or the number of cells per colony (Figure 2B, right panel) which are indicators for cell proliferation.
Here we present a systematic comparison between cultivation-based assessment of cell viability and cultivation-independent indicators for cell viability.
We chose to use the tomato polygalacturonase 1 (Tand1) and 4 (TApromotersoters ligated to a GUS reporter gene as indicators for cell separation in tomato (Hong et al. 2000).
This study contains a systematic comparison between cultivation-based assessment of cell viability and cultivation-independent indicators for cell viability by sampling and analyzing heat-exposed B. subtilis cells going through the transition from culturable to unculturable cells.
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