The use of a red-shifted fluorescent Ca2+ indicator enabled us to orthogonalize indicator excitation and bPAC activation (Stierl et al., 2011): after recording basal Ca2+ levels at 520 nm, we repeatedly switched between excitation of the Ca2+ indicator at 520 nm and activation of bPAC at 485 nm; finally, we challenged sperm with 8-Br-cNMPs or db-cAMP while exciting the Ca2+ indicator only.
Comparison of the results predicted by fully coupled THM analysis as well as partially coupled TH, TM and HM analysis, in terms of several predefined indicators, enables us to identify the couplings, which play a crucial role with respect to safety issues.
Thirdly, using E2F5 status in addition of other clinical indicators, enabled us to obtain extremely high accuracy in separation of malignant from benign cases that was not possible without E2F5.
We selected three health indicators, enabling us to compare the differences between the impacts of SES on different health indicators.
This temporal extension of information on policy indicators enabled us to relate contextual data to information on stressful work and labour market disadvantage reported during this period.
Such estimation, using sustainability indicators of hydropower projects, enables us to understand marginal trade-offs among economic, environmental and social objectives of hydropower development.
Whereas this part of our analysis focuses on each sector of the US economy in isolation, we subsequently construct an indicator from the CAPE ratio that enables us to perform valuation comparisons across sectors.
The results of this study and the comparison of different approaches used to build accumulation indicators has enabled us to better understand and interpret the accumulation and interrelation of these inequalities in a given territory and to partially overcome problems of using spatial aggregated data.
"This enables us to detect indicators across an incredible breadth of attacks so we can protect our customers effectively.
Therefore, we design a set of partial indicators which enable us to analyse the role of commercial revenues in the economics of ANSPs.
