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Battery-powered portable pH meters are widely used for field tests of the pH of soils; such tests may also be performed, less accurately, by mixing indicator dyes in soil suspensions and matching the resulting colours against a colour chart calibrated in pH.
Cell viability was assessed using the following indicator dyes, Commassie Blue, Alamar BlueTM, Neutral Red, MTT and WST-1.
Currently, we are working on indicator dyes for sodium and potassium ion to measure these ions in sweat.
For example, Trupp et al. have synthesized a series of hydroxyl-substituted azobenzene derivatives as indicator dyes for optically monitoring pH between 6 and 10[4].
Our results therefore comprehensively confirm that the indicator dyes used in this study are not appropriate for the quantitative toxicity assessment of carbon nanotubes highlighting the pressing need for the development of alternative screening techniques.
Textile indicator dyes can be covalently linked to fibres containing hydroxyl and amino groups, they withstand washing at alkaline pH of conventional washing agents for >50 times, they react fully reversibly and are unaffected by ironing (Lobnik 2012).
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The pH indicator dye 4-[4- 2-hydroxyethanesulfonyl -phenylazo]-2,6-dimethylphenol 4-[4- 2-hydroxyethanesulfonyl -phenylazo]-2,6-dimethylphenol 4-[4- 2-hydroxyethanesulfonyl -phenylazo]-2,6-dimethylphenol 4-[4- 2-hydroxyethanesulfonyl -phenylazo]-2,6-dimethylphenol 4-[4- 2-hydroxyethanesulfonyl -phenylazo]-2,6-dimethylphenol 4-[4- 2-hydroxyethanesulfonyl -phenylazo]-2,6-dimethylphenol 4-[4- 2-hydroxyethanesulfonyl -phenylazo]-2,6-dimethylphenol
The second viability assay employed the fluorogenic redox indicator dye, Alamar Blue.
The assay is based on reduction of the indicator dye, resazurin, to the highly fluorescent resorufin by viable cells.
To combine this analysis, we employed an independent method based on chemical reactivity with an indicator dye.
The assay is based on the reduction of the indicator dye, resazurin, to the highly fluorescent resorufin by viable cells.
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