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This metabolite has been proposed as an indicator compound for quality control due to its instability and rapid degradation when compared to other secondary metabolites within plant materials [9, 18].
The idea was to use an indicator compound for that purpose.
In the current Chinese Pharmacopoeia, chlorogenic acid is officially used as the indicator compound to characterize the quality of this herb [ 23].
An appropriate indicator compound should be prone to oxidation in ESI, and two forms should be detected by MS, a reduced and an oxidized form.
For characterizing the reducing capacity of an AOX, solutions of the AOX (0 1 mM) were mixed with an indicator compound (amodiaquine, 20 μM; morphine, 20 μM; melatonine, 20 μM) and analyzed in triplicate.
The addition of a redox-active compound should induce a decrease of the electrochemical potential, which should further give rise to a shift of signal intensity from the oxidized form of the indicator compound to its reduced form.
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The introduced measurement technique is applicable to mixing systems containing one compound, which potentially can form hydrogen bonds, such as water, alcohols or amines, and does not rely on the addition of traces of indicator compounds.
It is recalled that indicator compounds and intermediates such as SCFA and ammonia are produced by microbial activity.
To control the upgrading measures with sufficiently high resolution in time these indicator compounds are measured in 65 additional wastewater samples.
Additionally, some pharmaceuticals and the artificial sweetener acesulfame have been defined as indicator compounds with a constant discharge in recipient waters.
The IndVal method reveals 18 significant indicator compounds for G1 and seven for G2.
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