Exact(33)
As the toxicity indicator, cell viability or metabolic activity retention (R %) in the various biphasic systems was evaluated and the results were shown in Table 1.
The indicator cell line carries the HIV-2 long terminal repeat-driven green fluorescence protein (GFP) gene, which becomes activated upon infection with HIV or simian immunodeficiency virus.
Secondary viral infections were established in uninfected primary lymphocytes and indicator cell lines after their exposure to activated PBMCs, B cells, T cells, or plasma derived from CFS patients.
The TZM-bl indicator cell line is a HeLa cell derivative that expresses high levels of CD4, CCR5 and CXCR4.
The production of infectious virus in cells transfected with pLAI was confirmed by assays of the cell culture supernatant using the P4R5 indicator cell line (data not shown).
In all cases, the production of infectious virus in cells transfected with pLAI was confirmed using P4R5 indicator cell assays, as above (data not shown).
Similar(27)
The indicator cells form agglutinates (rosettes) around the antibody-bound Rh(D -positive fetal cells if present.
Typically, this assay is a two-step process involving (a) the activation of Th cells in vitro and (b) the testing of IL-2-dependent indicator cells for growth in the presence of Th cell supernatants.
Supernatants were harvested 48hrs post-transfection and indicator cells (TE671 cells) were incubated with different amounts of viral supernatant.
Viral titers were determined in single-round infectivity assays by applying filtered supernatant from producer cells on HeLa-CD4-LTRLacZ indicator cells.
After 24 hr, the supernatants were harvested and volumes corresponding to 5 ng p24Gag used to infect 105 TZM-bl indicator cells.
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