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JM101/L1.3, referred to as "wildtype" L1, contains a full-length copy of the L1.3 element and the mneoI indicator cassette cloned in pCEP4 (Invitrogen) [60], [63].
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Furthermore I confirmed by digestion with EcoRI that the experiment yielded approximately equal numbers of each orientation of the insertions (2 'A' isolates {Fig. 3c, lanes 3 and 6} and 4 'B' isolates {Fig. 3c, lanes 2, 4, 5, 7}. These experiments show that Flp can recombine a PCR fragment containing inverted FRT sites into a receptor vector containing an indicator lacZ FRT 2 cassette.
To further confirm that the Dicer is indeed required for controlling human L1 expression and retrotransposition, we introduced L1 expression cassettes harbouring a retrotransposition indicator for cell culture-based assay [ 11, 19].
For most reporters in this work, the internalization of the Cre was monitored by genomic excision of a "floxed" cassette, allowing the expression of various indicator genes.
Select music I own on vinyl, cassette, (never ever an 8-track - can you imagine?) disc and via download, the truest indicator of ongoing fanship over the years.
One indicator of how fast the upstart DVD is catching the elderly videocassette is the number of discs and cassettes manufactured each year.
An audio cassette tape.
On a cassette.
Recorded on a cassette.
Cassette tapes suck!
Except without cassette decks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com