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When we stained the FUS-MB treated brains with Alexa 488-conjugated goat anti-mouse IgG antibodies (Invitrogen, Eugene, OR, USA), we observed diffuse fluorescence throughout the sonicated volume, 10 to 20-fold greater than in the untreated hemisphere, indicative of endogenous mouse IgG that had extravasated across the BBB (Figure 3A).
A zone of TN-C, localised to the myoepithelial-gel interface was evident, indicative of endogenous basement membrane production.
We addressed this issue by monitoring replication protein A (RPA) focus formation in unstressed cells, which is indicative of endogenous ssDNA accumulation due to fork stalling and/or DNA damage.
Thus, hIL-6 detected in the serum and urine post-injection of hIL-6 is not indicative of endogenous (murine) production of IL-6, but does reflect the metabolism/elimination of circulating IL-6 in AKI.
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In contrast to the findings of this study, a few CPM studies in TMD and OA patients have shown impaired CPM effects indicative of dysfunctional endogenous analgesic systems [27, 29].
The persistence of behavioral, physiological, and/or gene expression rhythms under constant conditions is thus indicative of an endogenous clock.
The exclusive ectopic metaxylem which occurs in the hypocotyl of lax3, aux1-21 and their double mutant, and the prevalent ectopic metaxylem-like production of their TCLs, might be indicative of an endogenous IAA accumulation also in the latter mutants.
Therefore, high expression of GA biosynthetic genes is not necessarily indicative of higher endogenous bioactive GA levels and may instead result from their up-regulation under conditions of low GA.
These results suggested that the level of activity of the p38 MAPK pathway may be subject to the redox state of the cells and may be indicative of decreased endogenous oxidative stress associated with the longevity of this mouse [ 9, 13, 35, 37].
Plasma hyaluronidase levels (indicative of both endogenous hyaluronan- and glycocalyx-degrading capacity) were also increased in patients compared with controls (78 ± 4 vs 67 ± 2 U/ml, p < 0.05), which is in line with data from type 1 diabetes patients [ 10, 21].
Thus, the elevated levels of this complex are indicative of the decreased endogenous level of oxidative stress and may be a part of the mechanism of resistance to oxidative stress.
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