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In contrast, targeting of TIRC7 with sHLA-DR α2 led to a dramatic reduction of the cell number in G2/S phase as most of the cells prevailed in G0/G1 cell cycle phase (89%) (Figure 3A) which is indicative for cell cycle arrest.
These findings are confirmed by the lactate dehydrogenase (LDH) release assay which would be indicative for cell lysis.
However, it is also possible that the H2O2 accumulation in Pythium-infected rice roots was indicative for cell death upon nutrient depletion [ 21].
Prolonged incubation of endothelial cells with concentrations of up to 25 μg/mL papain did not result in increased release of lactate dehydrogenase which would be indicative for cell lysis.
Two seminal studies demonstrated that about 80% of primary myeloma samples display a gene expression signature characteristic of transcription factors of the nuclear factor κB (NF κB) family, and identified mutations indicative for cell autonomous activation of NF κB signaling in about 20% of myeloma cases.
Importantly, the fraction of non-nuclear localizations of the eGFP-anillin signal, such as contractile rings and midbodies (Fig. 8d), which are indicative for cell division [ 15] and were verified by staining for Aurora B kinase (Fig. 8e), was increased after miR-199 treatment compared to the control (Fig. 8f).
Similar(54)
The selection was performed based on colony morphology indicative for cells similar to murine ES cells.
Evaluation of nuclear morphology by fluorescence microscopy upon treatment with increasing drug-concentrations revealed Ukrain-triggered morphological alterations that are indicative for apoptotic cell death including chromatin condensation, nuclear shrinkage and fragmentation (Fig. 2a).
As the MLPA test enables detection of an aberration in a region covered by multiple MLPA probes if 20% or even less aberrant tumour cells are present (Hömig-Hölzel and Savola, 2012), we additionally included samples with a mean probe ratio of all DPYD probes below ⩽0.85 or above ⩾1.15, indicative for a cell fraction with deletion or duplication of the whole coding region.
Taken together, these data demonstrate that high PLAU mRNA levels are indicative for cancer cells that exhibit a functional TRAIL apoptotic signaling cascade, independently of their genetic background, tissue origin and the transformation events that led to the malignancy.
Although we measured a downregulation of Th1/Th17-associated cytokines and unaltered levels of Th2 cytokines after TCIT (Fig. 6), we also detected induction of IgG2a, indicative for B-cells switching under IFN-γ/Th1 influence.
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